Steps to take to complete ASR
Commercial biocatalysis requires robust enzymes that can withstand elevated temperatures and long incubations. Ancestral reconstruction has shown that pre-Cambrian enzymes were often much more thermostable than extant forms. Here, we resurrect ancestral enzymes that withstand ~30 °C higher temperatures and ≥100 times longer incubations than their extant forms.
Jupyter notebook to analyse the effect of data set size on ancestral predictions. Recreates figures from the GRASP paper.
Steps through the process of aligning sequences, inferring phylogenetic trees, and using GRASP to predict ancestors. Provides an overview of GRASP's features.
Detailed explanation of the features of GRASP and how to use it.
Jupyter notebook tutorials that give the user an environment to analyse and curate sequence data sets. Includes notebooks for automatic and iterative removal of sequences and notebooks to map and visualise exon structure on multiple sequence alignments.